Fig. 7

MVB-dependent degradation of EGF-R in fibroblasts from individuals with DS and euploid controls. a Western blot of EGF-R in euploid fibroblasts and fibroblasts from individuals with DS illustrating the degradation of the EGF-R at times 0, 30, 60, 90 and 120 min of EGF treatment. GAPDH is used for normalization; b Mean EGF-R level at time = 0 min is not significantly different between euploid (n = 3 individuals) and DS fibroblasts (n = 3 individuals) (three independent experiments each including one euploid and one DS individual) (Mann-Whitney test, p-value = 0.4); c Quantification of EGF-R level expressed as a percentage of EGF-R level at time = 0 min in each condition and normalized to GAPDH level shows that the degradation of EGF-R is significantly delayed in fibroblasts from individuals with DS as compared to euploid fibroblasts (Two-way ANOVA, *p-value = 0.012; post-hoc Bonferroni test, adjusted p-value at t_{30 min} > 0.99, *adjusted p-value at t_{60 min} = 0.018; **adjusted p-value at t_{90 min} = 0.0077, *adjusted p-value at t_{120 min} = 0.015)