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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome

Fig. 5

Confocal microscopy and SR-SIM of human isogenic iPSCs-derived neurons from an individual with T21 mosaicism. a Confocal z-projected representative images of DAPI (blue), MAP 2 (red), EEA1 (green) labelling in euploid and isogenic T21 iPSCs-derived neurons; b Quantification of the mean EEA1-positive puncta volume in confocal microscopy images reveals significantly increased volume in T21 neurons as compared to euploid isogenic neurons (mixed effects ANOVA, genotype *p-value = 0.03); c Quantification of EEA1-positive puncta number normalized to the ROI area shows a significant increase (Mann-Whitney test, **p-value = 0.0043); d Representative z-projected images of a T21 neuron imaged by confocal microscopy (left) and SR-SIM (right). A cluster of EEA1-positive puncta observed by SR-SIM appearing as enlarged puncta by confocal microscopy is magnified (scale bar = 500 nm); e Quantification of EEA1-positive puncta volume in SR-SIM images shows no significant difference (mixed effects ANOVA, genotype, p-value = 0.95); f The mean number of EEA1-positive puncta clusters per cell is significantly increased in T21 neurons as compared to euploid isogenic neurons (Mann-Whitney test, *p-value = 0.047)

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