Fig. 4

Co-localization of mutant ataxin-3 aggregates with cerebellar blood vessels of MJD transgenic mice. a Representative co-immunofluorescence images for mutant ataxin-3 aggregates (HA = hemagglutinin in green) and CoIV (CoIV = collagen IV in red), confirming the presence of mutant ataxin-3 aggregates within CoIV-positive cerebellar blood vessels (Scale bar = 200 μm). In b and c, ataxin-3 aggregates are shown in blood vessels of deep cerebellar nuclei (DCN) and of lobule 9, respectively. The images are representative of a group of 7 transgenic animals (Scale bars = 5 μm)