Fig. 3

Fibrinogen extravascular deposition in the cerebellum of MJD mice. a, b Representative co-immunofluorescence images of fibrinogen (in green) and CoIV (in red) in the cerebellum of wild-type (WT) and MJD transgenic mice at 16–17.5 months old. Fibrinogen extravasation was more abundant in transgenic mice, particularly in deep cerebellar nuclei (DCN) and lobule 8 and 9, respectively (Scale bars = 50 μm). c Representative images of the method of quantification of extravascular fibrinogen previously described in the Materials and Methods section. Vascular fibrinogen is shown in white (co-localization of magenta and yellow) and CoIV in magenta, whereas extravascular fibrinogen is seen in yellow. d Quantification of the surface area of extravascular fibrinogen showed a 2-fold statistically significant increase (Unpaired t test, P = 0.03) in fibrinogen deposition in cerebellum parenchyma when comparing transgenic and wild-type mice. Values of n = 4 transgenic and n = 4 wild-type animals are presented as mean ± SEM. e Representative images of CoIV staining in whole cerebellum with the same scale in both wild-type and transgenic images. f Quantification of CoIV surface area showed an 16% increase in MJD mice comparing to wild-type littermates (Unpaired t test, P = 0.03). Values of n = 6 transgenic and n = 9 wild-type animals are presented as mean ± SEM Unpaired t test with Welch’s correction, * P ≤ 0.05