Fig. 5
Insoluble SYNJ1 is increased in AD brains and is correlated to tau load. a Summary of the fractionation protocol used to obtain total, RIPA-soluble, RIPA-insoluble and sarkosyl-insoluble fractions. b SYNJ1 was significantly decreased in the total homogenate of AD T1 isocortex. c SYNJ1 was significantly decreased in RIPA-soluble fraction of AD brains. c’ There was a significantly higher level of SYNJ1 protein detected in the AD cases carrying APOEε4 allele(s) compared to the AD cases without APOEε4 allele. d SYNJ1 was increased in RIPA-insoluble fraction of AD brains. Lower MW SYNJ1 positive bands were detected approximately around 100 kDa, 80 kDa and 50 kDa in the AD brain. For b-d, T1 isocortex from control (n = 42) and AD (n = 50) including 2 FAD cases with APP or PSEN1 mutation were analysed. **p < 0.01, ***p < 0.001, by unequal variances t test. d’ There was a significant positive correlation between the levels of SYNJ1 protein and phosphorylated tau detected using PHF1 in the RIPA-insoluble fraction. (r2 = 0.1443, n = 92, p = 0.0002, by Pearson correlation test)