Fig. 3

Detection of SYNJ1 immunoreactivities in Hirano bodies and in plaque-associated dystrophic neurites in AD brains. a-c A double immunofluorescence labelling for SYNJ1 (a, green) and actin (b, red) confirmed that Hirano bodies were immunostained for SYNJ1 in pyramidal neurons of AD brains (c, merge). While SYNJ1 labelling was stronger in the centre of the Hirano bodies (a), actin labelling was stronger in the periphery of Hirano bodies (b). There were numerous smaller punctiform structures that were actin positive but SYNJ1 negative in AD brains (arrows). d-f A double immunofluorescence labelling for SYNJ1 (d, green) and PHF1 (e, red) does not show association of SYNJ1-positive globular structures and hyperphosphorylated tau in the dystrophic neurites surrounding senile plaques. g-l A double immunofluorescence labelling for SYNJ1 (g, j, green) and Synaptophysin (SY38, h, k, red) shows partial colocalization of SYNJ1 and Synaptophysin (arrowheads) in AD brain (g-i) and in 5XFAD mouse brains at 12 months (j-l). Some plaque-associated dystrophic neurites were yet devoid of SYNJ1 immunoreactivity (l, asterisk). Representative images of an APOEε3/3 AD case (a-i) and 5XFAD (j-l) are shown. Scale bar 10 μm for a-c and 40 μm for d-l