Fig. 5

Immunohistochemistry of Bassoon in cortex of R6/1 and WT animals. a Double labeling of 8, 16, 40 weeks R6/1 cortex. EM48 positive aggregates are beginning to form at 8 weeks. In 40-week-old R6/1, inclusions are clear and there is a strong colocalization of Bassoon with the aggregates (scale bar = 20 μm). b Double labeling of 8, 16, 40 weeks WT cortex. No positive signal of EM48 exhibits and Bassoon staining maintains unchanged at different ages of mice (Scale bar = 20 μm)