Fig. 2

Primary neurons expressing mutant huntingtin protein (69Q) down-regulated the Bassoon protein expression. a Immunofluorescence of the huntingtin protein levels in GFP labeled neurons. Mutant huntingtin (69Q) expression induced the huntingtin aggregations (arrow) located in nucleus (scale bar = 75 μm). b Mutant huntingtin (69Q) caused the reductions of the linear densities of Bassoon clusters, leading to Bassoon containing synapse loss (arrowheads) in the dendrites (Scale bar = 5 μm). The quantifications of the linear densities of Bassoon (c), and Bassoon containing mature synapses (d). *p < 0.05, **p < 0.01, ***p < 0.001, n = 58–68 segments/group, N = 20–22 cells/group, triplicate individual experiments, one way ANOVA test, followed by a Tukey’s multiple comparisons post-test