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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation

Fig. 5

AX004/IgG1 isotype is more effective than AX004/IgG4 in potentiating tau uptake by primary human microglia cells (a) Flow cytometry analysis of human adult microglia cultures isolated from different donors showed increased fluorescence intensity in cells incubated with tau+AX004/IgG1 or tau+AX004/IgG4 in comparison to cells incubated with tau alone (tau vs tau+AX004/IgG1, ****p < 0.0001; tau vs tau+AX004/IgG4, ***p = 0.0003 by one-way ANOVA, Tukey’s multiple comparisons test). Comparison of the isotype IgG1 vs IgG4 of AX004 confirmed that AX004/IgG1 is more effective in facilitation tau uptake by microglia than AX004/IgG4 (*p = 0.0341, n = 13, by one-way ANOVA, Tukey’s multiple comparisons test). The effect of isotype controls IgG1 and IgG4 to tau uptake promotion had no effect in comparison to tau alone (tau vs tau+IgG1, p = 0.9988, n = 9; tau vs tau+IgG4, p = 9438, n = 9; by one-way ANOVA, Tukey’s multiple comparisons test). b Increased fluorescence detected after tau uptake in samples treated with AX004/IgG1 or AX004/IgG4 was significantly higher compared to cells treated with isotype controls (tau+IgG1 vs tau+AX004/IgG1, ****p < 0.0001, n = 13; tau+IgG4 vs tau+AX004/IgG4, ***p = 0.0002, n = 13 by one-way ANOVA, Tukey’s multiple comparisons test)

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