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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation

Fig. 3

Humanized antibodies AX004/IgG1 and AX004/IgG4 and their mouse counterpart, antibody DC8E8, discriminate between pathological truncated tau protein and physiological tau. a Affinity of AX004/IgG1, AX004/IgG4 and DC8E8 antibodies was higher for truncated tau 151–391/4R than for the physiological tau 2N4R. The discriminatory potency of humanized AX004 (isotypes IgG1 and IgG4) is slightly higher than that of the original mouse DC8E8 antibody (EC50tau2N4R: AX004/IgG1 = 47.5 ng/ml, AX004/IgG4 = 53.8 ng/ml, DC8E8 = 53.5 ng/ml; EC50tau151–391/4R: AX004/IgG1 = 5.4 ng/ml, AX004/IgG4 = 4.7 ng/ml, DC8E8 = 7.1 ng/ml). b All tested antibodies AX004/IgG1, AX004/IgG4 and DC8E8 recognized a similar pattern of neurofibrillary pathology (neurofibrillary tangles and neuropil threads) at a similar level in human AD hippocampus (CA1). Scale bars, 100 μm

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