Fig. 1
From: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation
Mouse neonatal primary microglia express microglia-specific markers in culture. a Representative fluorescent photomicrographs showing cell morphology of cultured mouse neonatal primary microglia cells. Green, ionizing calcium-binding adaptor molecule 1 (Iba-1), and blue, DAPI nuclear staining. Scale bars, 50 μm. b Flow cytometry analysis confirmed purity of primary microglia culture. Histogram shows the binding for anti-CD11b compared to the binding for nonspecific IgG. Quantification of four independent microglia cultures confirmed that around 95% of cells were positive for microglia marker CD11b (mean +/− SEM). c Analysis of FcγRs confirmed that almost all microglia express FcγI receptor (CD64), FcγII and FcγIII receptor (CD16/32) (mean +/− SEM)