Fig. 3
From: Loss of homeostatic microglial phenotype in CSF1R-related Leukoencephalopathy
Analysis of sub-population of macrophages in HDLS. a & b Immunofluorescence double stain of CD68 (Alexa Fluor 568 (red)), and IBA-1 (Alexa Fluor 488 (green)) with DAPI nuclear staining (blue). White arrowhead indicates an example of an IBA-1, CD68 double labelled amoeboid macrophage. White arrow indicates an example of a CD68 positive, IBA-1 negative amoeboid cell. Scale bar = 50 μm. c & d Immunofluorescent double stain of CD68 (Alexa Fluor 568 (red)), and S-100 (Alexa Fluor 488 (green)), with DAPI nuclear staining (blue). White arrowhead indicates an example of a CD68, S-100 double labelled cell with nuclear and cytoplasmic S-100 expression. Asterisk indicates example of a CD68 positive, S-100 negative cell. Scale bar = 25 μm