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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Transmission of ALS pathogenesis by the cerebrospinal fluid

Fig. 6

Characterization of the NfL-RFP; hTDP43WT double transgenic mice. (a) Schematic representation of HA-RFP1-tagged murine Rpl10a construction under control of the NFL promoter. —pBluescript KS +/− plasmid I, intervening sequence (IVS) and II, SV40 polyA. (b- d). Panel representing DAPI (blue), neuron specific marker, NeuN (green) and RFP (red) in the cortical sections (d) from the wild type (b) and NFL-rRFP (c) mice. (e- h) Scheme for the generation of double transgenic NfL-RFP; hTDP43WT mice (e). (f) represents the genotypic confirmation of the double transgene expression in the mice. (g) and (h) represent the scheme for the experimental procedure and extraction of peptides from the neuronal ribosomes using the EDTA-Trap method

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