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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Transmission of ALS pathogenesis by the cerebrospinal fluid

Fig. 3

ALS-CSF induced neuronal death and neurofilament abnormalities. (a-d) Spinal cord sections stained and quantified with Cresyl violet (a, b, n = 3.) and NeuN (red) (c, d, n = 3), respectively. Scale bar = 50 μm (a) and 20 μm (c). (e-h) Expression patterns and quantification of the immunoblots for ChAT protein (e) as well as the neurofilaments NfL (f) NfM (g) and InA (h) in the spinal cord lysates (n = 3). (i) Spinal cord sections immunostained for NfL (green) peripherin (Prph, red) and DAPI (Blue). Note the depletion of NfL immunostaining in neurites and myelinated axons, and the co-accumulations of NfL and Prph (white asterisk, i) in neuronal cell bodies in ALS group. (j) represents the expression patterns and quantification for Prph in the immunoblots for spinal cord lysates. (n = 3), Scale bar = 20 μm. Data are mean ± SEM. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and **** p ≤ 0.0001) and fold changes are calculated when compared to NALS

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