Fig. 5
From: Restoration of miR-193a expression is tumor-suppressive in MYC amplified Group 3 medulloblastoma
Interaction network analysis of the pathways significantly enriched in the genes downregulated upon miR-193a expression, and the change in the expression levels of the core histone marks in the medulloblastoma cells upon miR-193a expression. a The 439 genes significantly downregulated (padj < 0.05, log2 fold change < 0.8) upon miR-193a expression were analyzed for their interaction and functional enrichment (padj < 0.01) in the pathways from the KEGG and Reactome database using the ClueGO application in the Cytoscape software. MiR-193a targets E2F1, MAX, KMT2A are highlighted in the interaction network. The fill color of the node and that of the label indicate the statistical significance of the enrichment of the pathway. The higher size of the node indicates a higher number of interacting genes. b. Western blot analysis of the histone marks, H3K4me3, H3K27ac, and H3K27me3 in the medulloblastoma cells. The numbers below each band indicate the fold change in the expression levels of the histone mark in the P1, P2 populations expressing miR-193a as compared to the vector control cells after normalization using the total histone H3 levels as the loading controls