Fig. 2

Transcriptional Profiling of Previously Identified Therapeutic Targets. a Previously identified therapeutic targets with transcriptions fold-change metrics for adult versus pediatric samples. A positive fold-change indicates enrichment in pediatric patients, and conversely a negative value indicates enrichment in adult patients. b Volcano plot for all transcripts with previously identified targets indicated by red arrows. The solid black horizontal line at y = 1 indicates the threshold for p-value significance (p < 0.1; y = −log(0.1) = 1). c-e Clustering paradigms utilized in dataset exploration. c Dendrogram yielded from the DIvisive ANAlysis (DIANA) hierarchical clustering algorithm with respect to only the twenty previously identified targets. d Dendrogram produced by the AGlomerative NESting (AGNES) hierarchical clustering algorithm using complete linkage with respect to only the twenty previously identified targets. e Clustering partitions and group ellipsoids generated by the fuzzy analysis (FANNY) probabilistic k-centroid technique with respect to only the twenty previously identified targets. f-h Silhouette plots depicting mean silhouette width across a range of numbers of clusters. f Silhouette plot generated from the DIANA clustering presented in 2c. g Silhouette plot produced by the AGNES algorithm output presented in 2d. h Silhouette plot yielded by the FANNY algorithm results visualized in 2e