Table 1 Table of species, concentration and source of the antibodies used for immunostaining
CD4 | T helper cells | Mouse | 1:2000 | AbD Serotec |
|---|---|---|---|---|
CD8 | Cytotoxic T cells | Mouse | 1:2000 | AbD Serotec |
CD79a1 | B cells | Mouse | 1:2000 | Thermoscientific |
IFNγ | Cytokine | Mouse | 1:100 | Santa Cruz |
TNF | Cytokine | Goat | 1:100 | R&D Systems |
Iba1 | Microglia/Macrophages | Rabbit | 1:2000 | Wako, Japan |
NeuN | Neuronal Nuclei | Mouse | 1:1000 | Chemicon |
MOG | Myelin/oligodendrocytes | Mouse | 1:50 | R. Reynolds |
CNPase | Myelin/oligodendrocytes | Mouse | 1:1000 | Millipore |
NFH | 200 kDa Neurofilament protein | Mouse | 1:1000 | Chemicon |
Cl-Casp3 | Apoptotic cells | Rabbit | 1:1000 | Cell Signalling |
MLKL | TNF signalling | Rabbit Mouse | 1:500 1:250 | Cell signalling Santa Cruz |
pMLKL | Necroptotic cells | Rabbit | 1:500 | Abcam |
RIPK3 | TNF signalling | Rabbit | 1:300 | Sigma |
pRIPK3 | Necroptotic cells | Rabbit | 1:500 | Abcam |
Olig2 | Oligodendrocytes | Rabbit | 1:500 | Abcam |