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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration

Fig. 6

Changes to gait parameters measured using Catwalk XT. Following 1 week of training, a quantitative fully automated gait analysis was conducted on the animals 7 days before surgery and then repeated at 9, 21 and 35 days post injection of the cytokine or GFP lentiviral vector. The graphs show the average for each parameter from 6 compliant runs with separate lines for each of the paws (RF: right forelimb; LF: left forelimb; RH: right hindlimb; LH: left hindlimb). The body speed was significantly lower in both IFA and MOG immunised animals at 21 dpi compared to GFP (a). Both IFA and MOG animals showed a decrease in percentage of the regularity index (%), which is the number of normal step sequence patterns relative to the total number of paw placements used as a measure of interlimb coordination (b). Cytokine viral vector injection decreased the duration of the stance phase, which is the average time in seconds that the paw is in contact with the glass plate for each step cycle, of all paws at 21 dpi and the hind paws at 35 dpi (c). The duty cycle represents the percentage of time the paw accounts for the total step cycle of the paw and decreased for the right front and left and right hind paws at 21 dpi for IFA animals and at right front and hind paws for MOG animals in animals receiving cytokine viral vectors (d). There was no change to swing speed following cytokine injection at any timepoint (e). All values are given as mean ± SEM. Statistics: ANOVA with TUKEY post hoc test. *P < 0.01 compared to GFP injected group

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