Fig. 6

Effect of AQP4 deficiency on neuroinflammatory responses in 5xFAD mice. a qPCR analysis of brain hemispheres from wild-type (black columns), 5xFAD (red columns), and 5xFAD/AQP4 KO (blue columns) mice. The level of the transcript of each gene in 5xFAD and 5xFAD/AQP4 KO mice was determined as the % of that of age-matched wild-type mice. Values are mean ± S.E.M of 5–6 individuals. *(P < 0.01) and **(P < 0.001) represent significant differences versus wild-type mice. The primers used are listed in Supplemental Table S2. b-g Immunostaining of Aβ (b and e), GFAP (c and f), and Iba1 (d and g) in the hippocampal region of 5xFAD/AQP4 KO (b-d) and 5xFAD (e-g) mice. Scale bars = 500 μm