Fig. 4
Effect of AQP4 deficiency on the deposition of Aβ in parenchyma of 5xFAD female mice. a-h Representative immunohistochemical images of sagittal brain sections of 40-week-old 5xFAD/AQP4 KO (a, b, e, and f) and 5xFAD (c, d, g, and h) mice stained with an anti-Aβ42 antibody. b, d, f, and h are magnified images of a, c, e, and g, respectively, indicated by boxes. Scale bars = 1 mm (a, c, e, and g) and 100 μm (b, d, f, and h). i and j Quantification of the number of amyloid plaques and plaque size in the cortical region. The average number of plaques (i) and plaque size (j) in ten fields in two sections of cortical regions from each animal (n = 3), including (a) and (c), were counted using ImageJ. Values are mean ± S.E.M of 3 individuals. k and l Quantification of the number of amyloid plaques of approximately 25 and 100 μm in diameter calculated by Imaris in the 3D imaging of cleared hemispheres from 45-week-old 5xFAD (red columns, Supplemental Movie S5) and 5xFAD/AQP4 KO (blue columns, Supplemental Movie S6) mice stained with Alexa Fluor 488-labeled 6E10 to identify total Aβ (k, green in movies) and Amylo-Glo to identify Aβ fibrils (l, magenta in movies). Values are mean ± S.E.M of 3 individuals. m and n The amounts of insoluble Aβ42 (m) and Aβ40 (n) extracted from the cerebral hemispheres of 5xFAD (red columns) and 5xFAD/AQP4 KO (blue columns) mice were determined by ELISA. Values are mean ± S.E.M of 6–7 individuals