Fig. 8

Unilateral intranigral administration of BSSG induces bilateral senescence in the SNpc. a Representative micrographs of double-stained slices with TH immunohistochemistry and β-Gal (TH + β-Gal) and their respective separated β-Gal staining (β-Gal). The opacity of TH immunoreactivity was decreased by 60% to allow visualization of β-Gal staining. The numbers at the left margin of every row indicate the day post-lesion. b The Graphs show β-Gal area density in the conditions of panel a. The bars stand for the mean ± S.E.M. calculated from the measurements in three anatomical levels. n = 3 independent rats per time point in the BSSG group; n = 6 for Mock and UT groups. *, BSSG group compared with the control groups. Two-way ANOVA, Bonferroni post-test. p < 0.05. Double stained slices with GFAP immunohistochemistry and β-Gal (c,e) and their respective separated β-Gal staining (d,f). Double stained slices with Iba1 immunohistochemistry and β-Gal (g,i) and their respective separated β-Gal staining (h,j). Red arrowhead shows ameboid microglia in senescence, and the blue arrowhead shows active microglia. k Astrocyte shape is illustrated in the conditions mock and l BSSG on day 60 after the lesion. The scale bar = 50 μm is common for all micrographs