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Fig. 10 | Acta Neuropathologica Communications

Fig. 10

From: Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicity

Fig. 10

Ceramide-enriched 5xFAD brain tissue and serum exosomes are neurotoxic. (a) Cluster analysis of wild type (WT) and 5xFAD brain tissue-derived exosomes after Nano Particle Tracking analysis. N = 3 (b) Immunoblot of exosome markers flotillin-1, Alix-1, CD9, and GFAP, demonstrating higher amounts of GFAP in 5xFAD exosomes compared to WT exosomes. c FLICA assay shows 3-fold increased rate of apoptosis induction (cells with activated caspase 3) after incubation for 12 h of N2a cells with 5xFAD brain tissue exosomes compared to WT exosomes. N = 15, Student t-test with Welch’s correction p < 0.005. d Cytotoxicity (CyQuant) assay shows reduction of neurotoxicity after depletion of ceramide-enriched exosomes. N = 7, Multiple Student t-test with Welch’s correction p < 0.05

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