Fig. 3

Analysis of cell types in cadaveric control and seizure-affected dentate gyri demonstrating GCD. (a-t) Immunohistochemistry studies were performed on coronal paraffin sections of control and seizure-affected cadaveric hippocampi using GC markers, namely PROX1, CTIP2, Calbindin, BLBP. Representative images of compact, tram-track (TT) and disaggregated (DA) DG from both control and seizure cases are demonstrated. No difference was observed between control and seizure brains with respect to molecular expression of PROX1, CTIP2, Calbindin and BLBP across groups. PROX1 was expressed in both inner and outer GC layers of the tram-track DG (a-c,m,n). CTIP2 and Calbindin expression is more prominent in the outer layer, compared to the respective inner layers, as seen in compact and tram-track DG (d,e,g,h,o,p); but was expressed in the entire DA zone both in control and seizure cases (f,i,p,r). BLBP marked the inner granular layer and the hilar region more densely, as expected (j-l,s,t). BLBP staining in SZ-1 showed some non-specific background due to presumed cell autolysis (s). TT, tram-track; arrowhead, outer granular zone distal to hilus; open arrowhead, inner granular zone proximal to hilus; bv, blood vessel. Scalebars: 50 μm (a-t)