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Table 2 Antibodies used for immunohistochemistry and for immunoblotting

From: Enhanced accumulation of N-terminally truncated Aβ with and without pyroglutamate-11 modification in parvalbumin-expressing GABAergic neurons in idiopathic and dup15q11.2-q13 autism

NameEpitope or targetDilutionHost/typeSource
6E104–10 aa Aβ1:4000M-monoclIBRDD [19, 26]
4G818–23 aa Aβ1:3000M-monoclIBRDD [19, 26]
MOAB-2a, not APP1:100M-monoclLifeSpan Biosciences, Inc., Seattle, WA
IBR226Aβ C-terminus 36–421:40R-polyclIBRDD [14]
12F4Aβ-42 C-terminus1:200M-monoclBioLegend, San Diego CA
RabmAb42Aβ C-terminus 36–421:100R-monoclIBRDD [34]
IBR162Aβ C-terminus 34–401:200R-polyclIBRDD [14]
R510Aβ-pE111:2000R-polyclIBRDD
R57APP aa 671–6951:2000R-polyclIBRDD [14, 15]
Anti-GAD65/67glutamic acid decarboxylase1:250R-polyclMillipore-Sigma, Burlington, MA
Anti-parvalbuminparvalbumin1:100S-polyclThermoFisher, Waltham, MA
Anti-parvalbuminparvalbumin1:20G-polyclNovus Biologicals, LLC, Centennial, CO
Anti-somatostatinsomatostatin1:100S-polyclNovus Biologicals, LLC, Centennial, CO
Ab-1cathepsin D1:200M-monoclEMD Biosciences, San Diego, CA
  1. a Unaggregated, oligomer and fibrillar Aβ, no cross-reaction with human APP
  2. The antibodies were monoclonal or polyclonal affinity purified mouse (M); rabbit (R) sheep (S) or goat (G)