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Table 2 Antibodies used for immunohistochemistry and for immunoblotting

From: Enhanced accumulation of N-terminally truncated Aβ with and without pyroglutamate-11 modification in parvalbumin-expressing GABAergic neurons in idiopathic and dup15q11.2-q13 autism

Name

Epitope or target

Dilution

Host/type

Source

6E10

4–10 aa Aβ

1:4000

M-monocl

IBRDD [19, 26]

4G8

18–23 aa Aβ

1:3000

M-monocl

IBRDD [19, 26]

MOAB-2

a, not APP

1:100

M-monocl

LifeSpan Biosciences, Inc., Seattle, WA

IBR226

Aβ C-terminus 36–42

1:40

R-polycl

IBRDD [14]

12F4

Aβ-42 C-terminus

1:200

M-monocl

BioLegend, San Diego CA

RabmAb42

Aβ C-terminus 36–42

1:100

R-monocl

IBRDD [34]

IBR162

Aβ C-terminus 34–40

1:200

R-polycl

IBRDD [14]

R510

Aβ-pE11

1:2000

R-polycl

IBRDD

R57

APP aa 671–695

1:2000

R-polycl

IBRDD [14, 15]

Anti-GAD65/67

glutamic acid decarboxylase

1:250

R-polycl

Millipore-Sigma, Burlington, MA

Anti-parvalbumin

parvalbumin

1:100

S-polycl

ThermoFisher, Waltham, MA

Anti-parvalbumin

parvalbumin

1:20

G-polycl

Novus Biologicals, LLC, Centennial, CO

Anti-somatostatin

somatostatin

1:100

S-polycl

Novus Biologicals, LLC, Centennial, CO

Ab-1

cathepsin D

1:200

M-monocl

EMD Biosciences, San Diego, CA

  1. a Unaggregated, oligomer and fibrillar Aβ, no cross-reaction with human APP
  2. The antibodies were monoclonal or polyclonal affinity purified mouse (M); rabbit (R) sheep (S) or goat (G)