Fig. 3

Control and dup-15 (d-15) sections double immunostained for GAD65/67 and for N-tr-Aβ (Aβ) with mAb 4G8 and counterstained with TOPRO-3i (TOPRO). The immunoreaction for N-tr-Aβ was more intense and with more numerous intracellular profiles in dup-15 subjects than in controls. The reaction for Aβ was mainly detectable in the GAD65/67-positive neurons in all studied groups as cytoplasmic granules, with a notable granular reaction in the nucleus, as shown in the magnified inset (d-15 – magn.). GABAergic neurons with a strong N-tr-Aβ immunoreaction typically showed a less intense reaction for GAD65/67. The scale bars show 20 μm