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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Tacr3 in the lateral habenula differentially regulates orofacial allodynia and anxiety-like behaviors in a mouse model of trigeminal neuralgia

Fig. 5

Chemicogenetic inhibition of unilateral LHb neurons alleviated pT-ION-induced anxiety-like behaviors but not allodynia. (a) The injection sites of pAOV-CaMKII-HM4Di virus. The green area with black dots shows the virus injection. (b) Typical immunofluorescence staining showing the location of virus transfection in the LHb. Green, EGFP; Blue, DAPI. (c) The time schedule of pAOV-CaMKII-HM4Di virus and CNO administration, behavioral testing, and PCR. (d) Primary mechanical allodynia (left panel), secondary mechanical allodynia (middle panel), and secondary cold allodynia (right panel) were unchanged by pAOV-CaMKII-HM4Di injection in the left LHb and the subsequent CNO application. ### p < 0.001 vs. the sham group. (e) The percentage of distance and entries but not time spent in the open-arm of the EPM was reversed by the inhibition of left LHb neurons by HM4Di-CNO. # p < 0.05 vs. the sham group; *p < 0.05, ** p < 0.01 vs. the pT-ION+HM4Di + Saline group. (f) The central distance, central time, and central/total distance in the OFT were significantly reversed by the inhibition of left LHb neurons by HM4Di-CNO. # p < 0.05, ### p < 0.001 vs. the sham group; *p < 0.05, *** p < 0.001 vs. the pT-ION+HM4Di + Saline group

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