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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Characterising cellular and molecular features of human peripheral nerve degeneration

Fig. 3

Immunohistochemical and RT-qPCR analysis of Schwann cells in healthy and denervated human nerves. a-c represent nerve cross sections immunostained for SOX10 (brown) and P75 p75NTR (red) along with haematoxylin and eosin stain. The black arrow in the micrographs indicates a SOX10/p75NTR positive Schwann cell. di the x-axis represents Log (denervation time in days). In d-g, the horizontal black dotted line represents the mean value obtained for the healthy nerve group. a Healthy sural nerve b Biceps branch of the musculocutaneous nerve denervated for 30 days. The brown staining represents a SOX10 positive nucleus whilst the red cytoplasmic staining represents p75NTR positive staining. c Axillary nerve denervated for 294 days with deteriorated morphology. d Scatter plot to represent the total number of haematoxylin positive cells/mm2 in denervated samples. e Nonparametric smoothing linear regression of the total number of haematoxylin positive cells/mm2 in denervated samples. f Scatter plot to represent the total number of Schwann cells/mm2 across denervated samples g Nonparametric smoothing linear regression of the total number of SOX10 positive Schwann cells/mm2h RT-qPCR analysis of SOX10 mRNA expression across denervated samples. i Non-parametric smoothing linear regression of the SOX10 RT-qPCR data. Case numbers are attached to each data point for reference to Table 1 with descriptors of whether the samples were collected proximally or distally: m1 - Proximal part of the denervated stump of the biceps branch of musculocutaneous nerve. m2 - Distal part of the denervated stump of the biceps branch of musculocutaneous nerve. b1 - Proximal section of the denervated stump of the brachialis branch of musculocutaneous nerve. s1 - Denervated stump of suprascapular nerve. sa1 - Proximal section of the denervated stump of the spinal accessory nerve. sa2 - Distal section of the denervated stump of the spinal accessory nerve

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