Table 2 Amino acid sequences of mimotopes, peptides, and their derivatives for immunization
From: Induction of aquaporin 4-reactive antibodies in Lewis rats immunized with aquaporin 4 mimotopes
Mimotope ID | Mimotope: Amino acid sequences including GGGS linker | Mimotope-KLH: Mimotope-keyhole limpet hemocyanin fusions for immunization protocol 1 | Mimotope-AQP4268–285: Mimotopes fused with AQP4-reactive T cell epitope used for immunization protocol 2 |
|---|---|---|---|
I-04 | WRYHVHPTPFKSGGGS | WRYHVHPTPFKSGGGSC- keyhole limpet hemocyanin | WRYHVHPTPFKSKAAQQTKGSYMEVEDNRS |
I-13 | GPFHFLHHHWSQGGGS | GPFHFLHHHWSQGGGSC- keyhole limpet hemocyanin | GPFHFLHHHWSQKAAQQTKGSYMEVEDNRS |
I-18 | WSSHAHRHNHFRGGGS | – | |
IV-04 | WPWHAHGNTRGIGGGS | WPWHAHGNTRGIGGGSC- keyhole limpet hemocyanin | WPWHAHGNTRGIKAAQQTKGSYMEVEDNRS |
IV-27 | IQYAPGGSYSVIGGGS | – | |
IV-38 | VKGHWHHLNHANGGGS | – | VKGHWHHLNHANKAAQQTKGSYMEVEDNRS |
II-01 | FPFWHRTHAWDRGGGS | – | |
III-01 | WSWKHHHPIMPRGGGS | WSWKHHHPIMPRGGGSC-keyhole limpet hemocyanin | WSWKHHHPIMPRKAAQQTKGSYMEVEDNRS |
“mix”: I-04 (WRYHVHPTPFKSGGGSC- keyhole limpet hemocyanin) + I-13 (GPFHFLHHHWSQGGGSC- keyhole limpet hemocyanin) + IV-04 (WPWHAHGNTRGIGGGSC- keyhole limpet hemocyanin) + III-01 (WSWKHHHPIMPRGGGSC-keyhole limpet hemocyanin) | – | ||
Neg.controls: | – | – | |
II-17 | HFWGHHRTTSKVGGGS | – | – |
random peptide L5 | SPRAISSYPLNEGGGS | – | – |
random peptide L8 | FPTDSLRGDVGMGGGS | – | – |