Fig. 4

Orientation of mitotic spindles (upper panel) and TPX2 immunoreactivity in mitotic astrocytes (lower panel). a, b Mitotic spindles are oriented along the long cellular axes. Note that: 1) immunoreactivity for gamma-tubulin (γ-tub) is localized in centrosomes (arrows in a’ and b′) and at the poles of the spindles; 2) in (b) that one centrosome is displaced into a proximal part of a process (arrow in b) and that this centrosome co-localizes with TPX2 in the process; 3) areas occupied with spindles are devoid of GFAP immunoreactivity. For an animated 3D reconstruction of the spindles see Additional files 4a and 4b. c, d The mitotic spindle is oriented perpendicular to the long cellular axis. Note the widening of the spindle poles, the appearance of additional foci of gamma-tubulin reactivity, and lagging chromosome (arrow) in c′d’). For an animated 3D reconstruction of the spindle in (c) see Additional file 4C. e, f Metaphase chromosomes located at the periphery of cell body. Note several foci of gamma-tubulin immunoreactivity and lagging chromosomes (arrows) in (e’,f’). For an animated 3D reconstruction of the spindles see Additional files 4E and 4F. g-i TPX2 immunoreactivity in mitotic astrocytes is present in cell body and processes indicating penetration of astral microtubules in processes. g Prophase. g’ shows two channels TPX2 and γ-tubulin. Centrosomes are indicated with arrows. h Metaphase. i Anaphase. Lower right insets in (h and i) show chromosomes. All images were obtained with confocal microscope. Scale bars: a-f = 10 μm,  g-i = 15 μm