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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: A high–throughput digital script for multiplexed immunofluorescent analysis and quantification of sarcolemmal and sarcomeric proteins in muscular dystrophies

Fig. 5

a Percentage of fibres in each sample that were classified positive for the presence of fetal and developmental myosin. All samples were immunostained and acquired at the same time under the same conditions. N = 2 serial sections for each sample. b Image panel highlighting f/d myosin (pink 647) and lamininα2 staining (red 568) of CTRL_1, BMD_3 and DMD_3 and their corresponding f/d myosin classification maps generated via digital analysis. Blue fibres in the classification map are negative for f/d myosin whilst red fibres have been classified as positive

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