Fig. 4From: A high–throughput digital script for multiplexed immunofluorescent analysis and quantification of sarcolemmal and sarcomeric proteins in muscular dystrophiesMean fluorescence intensity of sarcolemmal α-sarcoglycan (a) and β-dystroglycan (d). Cumulative fluorescence intensity for α-sarcoglycan (b) and β-dystroglycan (e). Average % myofibres positive for α-sarcoglycan (c) and β-dystroglycan (f). A positive fibre is classified as having > 25% sarcolemmal protein circumference coverage. β-dystroglycan (g) and α-sarcoglycan (h) intensity in regions of sarcolemmas that were classified as either positive or negative for dystrophin. All samples were immunostained and acquired at the same time under the same conditions. N = 2 serial sections for each sampleBack to article page