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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: A high–throughput digital script for multiplexed immunofluorescent analysis and quantification of sarcolemmal and sarcomeric proteins in muscular dystrophies

Fig. 3

a Myofibre classification key for coverage of sarcolemmal protein markers (Dystrophin, α-sarcoglycan or β-dystroglycan). Sarcolemmal circumference coverage is used to place fibres in 4 categories. Protein negative (< 25% coverage) or varying degrees of protein positive (25–50%, 50–75% or 75–100% sarcolemmal circumference coverage). b Image panel highlighting dystrophin immunofluorescence staining (green 488) of CTRL_1, BMD_3 and DMD_3 and their corresponding dystrophin classification maps. Yellow regions show connective tissue or myofibres have not been recognised/detected during the fibre recognition phase

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Acta Neuropathologica Communications

ISSN: 2051-5960

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