Fig. 1From: A high–throughput digital script for multiplexed immunofluorescent analysis and quantification of sarcolemmal and sarcomeric proteins in muscular dystrophiesSerial sections from CTRL_1 were immunostained for dystrophin (a-b), a-sarcoglycan (c-d) and B-dystroglycan (e-f) separately on different occasions over a 5-week period. Each slide was immunostained according to the same protocol and acquired at the AxioScan with the same exposure times and configurations. Images were processed with the script to assess natural variability in immunostaining, acquisition and analysis. Fluorescence intensity and % positive myofibres were calculated for all images. Serial sections from CTRL_1 were also stained for f/d myosin to determine if false positive myofibres were being incorrectly identified during the analysis (g)Back to article page