Fig. 4
ANXA2–OSMR–STAT3 regulation and activation in GBM cells under normal and hypoxic conditions. a GSEA of IL6–JAK–STAT3 signaling genes and ANXA2 or OSMR expression in the GSE4412 dataset (n = 85). b, c Western blot analysis of the indicated proteins in MGG18 cells after ANXA2 (b) or STAT3 (c) knockdown. d, e Western blot analysis of the indicated proteins in MGG23 cells overexpressing ANXA2 with or without concomitant STAT3 (d) or OSMR (e) knockdown. f GSEA of hypoxia-associated genes and ANXA2 or OSMR expression in the GSE4412 dataset. g–j Western blot analysis of the indicated proteins in MGG23 cells overexpressing ANXA2 with or without concomitant OSMR knockdown. Cells were incubated in 1% O2 (g, h) or treated with deferoxamine mesylate (DFO) (i, j) for 24 h