Fig. 3

ANXA2- and OSMR-mediated regulation of angiogenesis in vitro. GSEA of angiogenesis-related genes and ANXA2 or OSMR expression in the GSE4412 dataset (n = 85). b ELISA analysis of VEGFA concentrations secreted by the indicated MGG23 cells incubation for 24 h in neural basal medium without growth factors (n = 8). c–e Tube formation assays of HUVECs after incubation for 20 h in growth factor-reduced Matrigel and conditioned media from the cells shown in (b). Representative images are shown in (c) and quantification of tube number and tubule length are shown in (d) and (e), respectively (n = 4). Scale bar, 500 μm. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Bonferroni’s post hoc test