Fig. 4

Mitochondrial respiratory chain deficiency in sCJD temporal cortex. a Representative photographs of mitochondrial respiratory chain complexes I-V staining (complex I: NDUFB8, complex II: SDHA, complex III: UQCRC2, complex IV: MTCO1, complex V: ATP5A) and VDAC1 in the temporal cortex of controls (top row), sCJD molecular subtypes VV2 (middle row) and MM1 (lower row). Evidence of MRC deficiency is observed in both MM1 and VV2 (+: positive neurons, #: deficient neurons). Scale bar: 50 μm. b Scatter plots showing the fraction of deficient neurons in the temporal cortex. Each dot represents the mean percentage of deficient neurons for each respiratory complex per individual with sCJD (VV2 or MM1) or control. Bars show mean and 95% confidence intervals