Fig. 2
Effect of formic acid treatment on immunohistochemistry. Formic acid treated (FA+) and untreated (FA-) tissue from disease-controls was compared using two different approaches. Findings from analyses in the temporal cortex are shown. a Semi-quantification of neuronal cytoplasmic stain, here exemplified by complex I (NDUFB8), shows less MRC deficiency in FA+ compared to FA- from the same three individuals. b Visual analysis and categorization of the same sections and individuals resulted in more consistent results. % deficient cytoplasm: mean percentage of neuronal unstained cytoplasm, % deficient neurons: percentage of total neurons from 2.5 mm2 of temporal cortex that did not stain positive. Bars show mean and range