Fig. 3
Effect of Temsirolimus treatment in vitro. a TMA staining of human tumor samples for HIF1-α and Pho-p70S6k. (n = 27, 17 KLF4wt, 10 KLF4K409Q). b Colony formation assay (CFA) and size analysis after Temsirolimus treatment. (n = 60). c Confocal imaging of actin stained IOMM cells. d RT-qPCR analysis of HIF1-α dependent genes after treatment with Temsirolimus under normoxic conditions. (n = 3). e Western Blot analysis of HIF1-α and (Ph)-p70S6K protein levels after treatment with Temsirolimus under hypoxia