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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: KLF4K409Q–mutated meningiomas show enhanced hypoxia signaling and respond to mTORC1 inhibitor treatment

Fig. 2

Effects of the KLF4K409Q mutation on cell viability, growth rate and hypoxia response. a Western blot analysis of KLF4 overexpression in transfected IOMM cells. b Colony formation assay (n = 60), cell viability assay after 4, 24 and 48 h (n = 5 / timepoint) and cell proliferation assay (n = 15) of KLF4wt- and KLF4K409Q -cell lines. c, d Western Blot and RT-qPCR (n = 3) analyses of cell lines under normoxic and hypoxic conditions. e Correlation of KLF4-mRNA and mRNA levels of hypoxia dependent genes in patient derived tumor samples. f Quantification of relative KLF4wt/K409Q protein stability with GFP-KLF4 constructs and blocking of protein translation with emetin at specified timepoints. (n = 3). g Quantification of hydroxylation dependent HIF1-α degradation in IOMM-KLF4wt/K409Q cell lines after transient transfection of HIF1-ODD-Luc (n = 3). h Western Blot analysis of hydroxylated HIF1-α after proteasome inhibition with MG132

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