Fig. 1

KLF4^K409Q Mutation results in a significant shift of gene expression and upregulates hypoxia driven pathways. a Transcriptomic analysis of 7 KLF4^K409Q and 10 KLF4^wt tumors. Nineteen thousand seven hundred ninety-nine protein-coding transcripts were considered, Trimmed Mean of M-value-normalization of all data. False discovery rate (FDR) < 0.1. Heatmap indicates the NES values derived for indicated hallmark gene sets by GSEA. Only gene sets with an FDR < 0.05 were considered. b Selective heatmap of genes relevant to hypoxia/glycolysis pathways. Red indicates significantly (FDR < 0.05) upregulated genes in KLF4^K409Q tumors, bold is upregulated but not significant. c Boxplots of KLF4, VEGF-A, SLC2A3 and HK2 expression within the analyzed samples. d, e Exemplary Western blot, its quantification of HK2 and TMA-IHC (e) of patient derived tumor-tissue confirming the increased expression of hypoxia dependent genes as well as KLF4 on the protein level in KLF^K409Q Meningiomas. (Western blot: n = 8, 4 KLF4^wt and 4 KLF4K409Q, TMA: n = 27, 17 KLF4^wt and 10 KLF4^K409Q)