Table 1 Primary and secondary antibodies
From: Loss of BICD2 in muscle drives motor neuron loss in a developmental form of spinal muscular atrophy
Epitope | Antibody | Concentration | Species |
|---|---|---|---|
Type 1 muscle fibres | BA-D5 (DSHB) | 1 in 100 | Mouse IgG2b |
Type IIA muscle fibres | SC-71 (DSHB) | 1 in 100 | Mouse IgG1 |
Type IIB muscle fibres | BF-F3 (DSHB) | 1 in 100 | Mouse IgM |
Laminin | L9393 (Sigma-Aldrich) | 1 in 100 | Rabbit |
Mouse IgG2b | AlexaFluor 488 (Life Sciences) | 1 in 500 | Goat |
Mouse IgG1 | AlexaFluor 647 (Life Sciences) | 1 in 500 | Goat |
Mouse IgM | AlexaFluor 568 (Life Sciences) | 1 in 500 | Goat |
Rabbit IgG | AlexaFluor 410 (Life Sciences) | 1 in 500 | Goat |
Mouse IgG | AlexaFluor 488 (Life Sciences) | 1 in 250 | Goat |
2H3 | DSHB (Supernatant) | 1 in 50 | Mouse IgG1 |
SV2 | DSHB (Supernatant) | 1 in 100 | Mouse IgG1 |
|  | Tetramethylrhodamine-bungaratoxin (−BTX, Cambridge Bioscience BT00012) | 1.5 μg/ml |  |
NF200 | N0142, SIGMA | 1 in 500 | Mouse IgG |
Peripherin | Merck Millipore, AB1530 | 1 in 500 | Rabbit IgG |
VSV-G | 8G5F11, Kerafast | 1 in 2000 | Mouse IgG |