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Table 1 Primary and secondary antibodies

From: Loss of BICD2 in muscle drives motor neuron loss in a developmental form of spinal muscular atrophy

Epitope Antibody Concentration Species
Type 1 muscle fibres BA-D5 (DSHB) 1 in 100 Mouse IgG2b
Type IIA muscle fibres SC-71 (DSHB) 1 in 100 Mouse IgG1
Type IIB muscle fibres BF-F3 (DSHB) 1 in 100 Mouse IgM
Laminin L9393 (Sigma-Aldrich) 1 in 100 Rabbit
Mouse IgG2b AlexaFluor 488 (Life Sciences) 1 in 500 Goat
Mouse IgG1 AlexaFluor 647 (Life Sciences) 1 in 500 Goat
Mouse IgM AlexaFluor 568 (Life Sciences) 1 in 500 Goat
Rabbit IgG AlexaFluor 410 (Life Sciences) 1 in 500 Goat
Mouse IgG AlexaFluor 488 (Life Sciences) 1 in 250 Goat
2H3 DSHB (Supernatant) 1 in 50 Mouse IgG1
SV2 DSHB (Supernatant) 1 in 100 Mouse IgG1
  Tetramethylrhodamine-bungaratoxin (−BTX, Cambridge Bioscience BT00012) 1.5 μg/ml  
NF200 N0142, SIGMA 1 in 500 Mouse IgG
Peripherin Merck Millipore, AB1530 1 in 500 Rabbit IgG
VSV-G 8G5F11, Kerafast 1 in 2000 Mouse IgG
  1. DSHB Developmental Studies Hybridoma Bank