Fig. 2

EWAS results. a Manhattan plot showing the distribution of P values for differences between multiple system atrophy (MSA) patients and normal controls (CTRLs) along the chromosomes. The horizontal red line indicates the adj. P value cut-off, the horizontal blue indicates cut-off for FDR < 0.20. b-c Box plots showing b the 5-methylcytosine (5mC) or c 5-hydroxymethylcytosine (5hmC) levels of MSA patients and CTRLs. Boxes show 1st (Q1) to 3rd (Q3) quartile of data, the horizontal line shows the median, and the whiskers show Q1-inter quartile range (IQR; lower whisker) or Q3 + IQR (upper whisker). Outliers are shown with dots. d Correlation of 5mC and 5hmC beta values. MSA are green, CTRLs red. e Box plot showing the 5mC/5hmC ratio