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Table 2 Common testing techniques utilized to molecularly characterize pLGG including their associated cost, input requirements, limitations and time requirements

From: Pediatric low-grade glioma in the era of molecular diagnostics

TechniqueTime (hours)Cost (per sample)InputUtilityClinical Limitations
Immunohistochemistry++1 FFPE slide1 target/slide• Subject to antibody availability
Fluorescent in situ hybridization++++1 FFPR slide1 target/slide• Subject to probe design/ availability
Droplet digital PCR++10-50ng DNA/target1 target per reaction• Requires access to expensive equipment
NanoString nCounter++++200-500ng RNAUp to 800 targets per reaction• Requires RNA <10 years old
• Requires access to expensive equipment
SNP Array++++++100-200ng of DNADependent on probe frequency• Limited to copy number alterations
• Subject to batch effect
Next Generation Sequencing Panels++++++20-100ng DNA/RNADesign dependent• Requires RNA <10 years old
• Requires access to expensive equipment
• Requires significant downstream analysis
Methylation Array++++++20-50ng of bisulphite converted DNAMethylation-based diagnosis [20]• Requires access to expensive equipment
• Subject to batch effect