Fig. 4
From: Astrocytes rescue neuronal health after cisplatin treatment through mitochondrial transfer
Role of Miro-1 in mitochondrial transfer from astrocytes to neurons. Astrocytes were transfected with mito-GFP and Miro-1 siRNA or control scrambled (Scr) siRNA cultured with or without cisplatin 24 h and co-cultured for 17 h with neurons treated with or without cisplatin. Using confocal microscopy the percentage of neurons containing GFP was quantified. Data represents the mean ± SEM of 3 independent experiments performed in duplicate. Inset: Western blot to confirm knockdown of Miro-1 in astrocytes after transfecting with mito-GFP, Scr and Miro-1 siRNA. The percentage of knockdown was normalized to the control and quantified. Two-way ANOVA cisplatin x transfected astrocyte interaction: ****p < 0.0001, followed by Tukey’s post-hoc test: **p < 0.01, ****p < 0.0001