Fig. 4

Altered levels of the mitochondrial proteins ANT1 and VDAC1 in Tg mice. Representative images of sagittal CA1 sections from 6- (a), and 12-mo (b), Tg and Wt mice hippocampi. The sections were labeled using TOC1 (against soluble tau oligomers) (a), pathological phosphorylated tau AT8 (against the phosphorylated tau at Ser202/Thr205) (b), and anti-ANT1 antibodies (n = 3 for each mouse category). Immunofluorescence signals were analyzed using laser scanning confocal microscopy (z projection). Nuclei were visualized with DAPI staining. The scale bars represent 20 μm. The cellular ANT1 fluorescence intensity was quantified within CA1 cells from 6- and 12-mo Wt and Tg hippocampi (cells: 6-mo Wt, n = 106; 6-mo Tg, n = 52; 12-mo Wt, n = 63; 12-mo Tg, n = 55). Graph shows the mean of cellular fluorescence per mouse category. Data are presented as mean ± SEM (*P < 0.05). Representative images of sagittal CA1 sections from 6- (c) and 12-mo (d) Wt and Tg mice hippocampi. The sections were labeled with antibodies against phosphorylated tau (pThr212), and VDAC1 (n = 3 for each mouse strain). Immunofluorescence signals were analyzed using laser scanning confocal microscopy (z projection). Nuclei were detected with DAPI staining. The scale bars represent 20 μm. The cellular VDAC1 fluorescence intensity was quantified within CA1 cells (6-mo Wt, n = 50; 6-mo Tg, n = 41; 12-mo Wt, n = 90; 12-mo Tg, n = 118). Graph shows the mean of cellular fluorescence per mouse category. Data are presented as mean ± SEM (**P < 0.01)