Fig. 6

N-acetylcysteine does not change the chemoattracting activity of the choroidal epithelium towards neutrophils. a Chemotaxis of PMNs towards the CPEC-conditioned medium. CPECs were treated with P3C or P3C + NAC for 14 h and conditioned medium was recovered from the apical compartment. Neutrophils were isolated from P3C-treated rats, added in the upper chamber of cell-free filters and allowed to migrate towards CPEC-conditioned medium in the bottom chamber. Migrated cells were recovered and counted. Data from individual filters, and mean ± SEM are shown as percentages of the mean value calculated for P3C-treated cells. The difference between the 2 groups was not statistically significant, one-tailed Student t test for unequal variance. b Effect of P3C and NAC on chemokine secretion by CPECs. Choroidal epithelial cell monolayers were treated with saline, P3C or P3C + NAC for 14 h. Media were sampled from both apical and basolateral compartments. Data are expressed as ng per filter and are shown as mean ± SEM (n = 5). For all five chemokines, secreted amounts in the P3C and P3C + NAC groups were statistically different from the saline group for both apical (filled) and basolateral (hatched) mediums, p < 0.001, one-way ANOVA followed by Tukey’s multiple comparisons test. There was no difference between the P3C and P3C + NAC groups