Fig. 2

Exposure of the choroidal epithelium to P3C induces a selective migration of neutrophils across the blood-CSF barrier. a Schematic representation of the experimental setup for the transmigration assay. Choroid plexus epithelial cells are cultured on the lower side of microporous filters and are pretreated with P3C or saline. White blood cells isolated from P3C-treated animals are added to the stroma-mimicking basolateral medium in the upper chamber of the device and allowed to transmigrate for 5 h. Migrated cells are recovered from the CSF-mimicking apical medium in the lower chamber. b Effect of P3C on the expression level of TLR2 gene in CPECs. Cells were treated with saline or P3C for 14 h. Data are shown as mean ± SEM (n = 6 from two different CPEC cell preparations). c Effect of P3C on leukocyte migration across CPEC monolayers. Data are shown as mean ± SEM (n) from two different CPEC and white blood cell preparations