Fig. 2

Further investigation of SNCA gains. a, b. Investigation of possible correlations of the level of mosaicism. Mosaicism relation to age of death in PD cingulate cortex (a), and to age of onset in MSA-SND (b). Best-fit line is shown for each cell type. Further details in text and Additional file 3: Table S3. c, d Combinations of FISH and α-synuclein IHC in DA neurons (identified by neuromelanin in brightfield) in ILBD (c), and in oligodendrocytes (identified by olig2) in MSA-SND (d). In both cases, cells with inclusions are shown, without SNCA gains at the top, and with gains at the bottom. Scale bar (c) 10 μm, (d) 5 μm. Note that the reference FISH probe was not used where olig2 was used