Fig. 7

Reporter experiments indicate that Egr-1 may trigger Cacna1c transcription in lumbar DRG of rats that received perioperative SD. a Representative fluorescence images showing that L-type channel (Cacna1c) is co-expressed mostly with Egr-1. b Western blotting of L-type channel in the L4–6 DRG of rats in the four groups at 9 days after surgery. c Quantitation of the results of B (10 rats per group). One-way ANOVA followed by a post hoc Tukey test: F (3, 4) = 17.13, **p < 0.01 for comparison of two groups. d Cacna1c gene promotor activity in HEK-293 T cells transfected with the empty GV141 vectors or the Egr-1 overexpression plasmids (3 replicates per treatment). Two tailed unpaired t test: t = 54.06, **p < 0.01 for comparison of the two groups