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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Sleep deprivation of rats increases postsurgical expression and activity of L-type calcium channel in the dorsal root ganglion and slows recovery from postsurgical pain

Fig. 6

Specific knockdown of L-type HVA channels in the lumbar DRG decreases the level and activity of HVA calcium channels and hyperexcitability of large DRG neurons on day 9 after surgery in rats subjected to perioperative SD. a Neurons labelled with EGFP indicated successful virus transfection. b Representative trace of total HVA calcium channels before and after bath perfusion of 1 μm nifedipine in large DRG neurons. c Current density of total HVA calcium channels before and after bath perfusion of 1 μm nifedipine (5–6 rats per group; 17 neurons from incision+SD + control group; 15 neurons from incision+SD + shRNA group). Two-tailed unpaired Student’s t-test: t = 3.169, **p < 0.01 for comparison of the two groups. d Effect of nifedipine on percentage change in current density. Numbers of neurons and rats were the same as in C. Unpaired Student’s t-test: t = 2.497, *p < 0.05 for comparison of the two groups. e Representative traces of evoked action potentials from DRG neurons. f Resting membrane potentials (RMP, left) and current threshold (CT, right) of the first action potential generation in large DRG neurons (5 to 6 rats per group; 18 neurons from incision+SD + control group; 13 neurons from incision+SD + shRNA group). Two-tailed unpaired Student’s t-test: tRMP = 2.959, tCT = 5.283, **p < 0.01 for comparison of the two groups. g Effect of stimulation intensity on the number of evoked action potentials. Numbers of neurons and rats were the same as in F. Two-way ANOVA followed by a post hoc Tukey test: Fgroup (1, 406) = 118.6. *p < 0.05, **p < 0.01 for comparison of the two groups at each stimulation intensity

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